Regulatory processes and population cyclicity in laboratory populations ofAnagasta kühniella (Zeller) (Lepidoptera: Phycitidae) IV. The sequential steps in the behavioral process of host finding and parasitization by the entomophagous parasite,Venturia canescens (Gravenhorst) (Hymenoptera: Ichneumonidae)

Summary The sequential steps in the behavioral process of a California stock of the entomophagous parasite,Venturia canescens (Grav.), parasitizingAnagasta kühniella (Zeller) was studied. In host-habitat finding, food media infested with hosts were very attractive to the parasites. Host finding was not covered in detail in this paper as it is presented in subsequent papers. Briefly, in all experiments host density was the most influential factor affecting the efficiency of the parasite. When three age stages of the host were exposed to a parasite, all tests showed that the large, last instar larvae was the preferred age stage but it was not the most suitable (when parasitized) for successful parasite development. Small larvae were less preferred but more suitable for parasite development when accepted. These studies were conducted as a partial fulfillment in the Ph. D. program of one of us (B. M. Matsumoto) and form a part of a broad investigation into the processes operating in the dynamics of arthropod populations under grants toC. B. Huffaker from the U. S. Public Health Service, National Institutes of Health and the U. S. Department of Agriculture.     

Isolation of D-4-chlorotryptophane derivatives as auxin-related metabolites from immature seeds of Pisum sativum

Summary Three active compounds which induce hypocotyl swelling in Phaseolus mungo were isolated from immature seeds of Pisum sativum. For purification of the compounds, our three-step procedure (counter-current distribution, Sephadex LH 20, and partition chromatography on silicic acid) were used. The chemical structures of two of the compounds were determined by synthesis to be -N-carboethoxyacetyl- and -N-carbomethoxyacetyl-D-4-chlorotryptophane. D-4-Chlorotryptophane is the first naturally occurring chlorinated amino acid found.     

Studies of Electric Capacitance of Membranes: I. A Model Membrane Composed of a Filter Paper and a Lipid Analogue

A hydrophobic filter paper of a given pore size containing a synthetic lipid, i.e. dioleyl phosphate, was interposed between aqueous electrolyte solutions having the same chemical composition and temperature. The electric capacitance and conductance of the membrane immersed in various concentrations of KCl were measured in the frequency range from 20 to 3 × 10⁶ cycle/sec. The observed capacitance and conductance were found to be strongly dependent on the applied frequency. A theory is proposed to account for this dispersion of impedance observed in the present membrane-electrolyte system. The dispersion is attributed to the formation of bilayer membranes of the lipid inside the filter paper. The effects of the salt concentration, the adsorbed quantity of the lipid, and the pore size of the filter paper on the capacitance and conductance of the membrane are discussed in terms of the distribution function of bilayers formed within the filter paper.     

The effect of photosynthetically active radiation and temperature on the photosynthesis of two Vietnamese species of Sargassum,S. mcclurei and S. oligocystum,based on the field and laboratory measurements

SUMMARY The effects of photosynthetically active radiation (PAR) and temperature on the photosynthesis of two Vietnamese brown algae, Sargassum mcclurei and S. oligocystum (Fucales), were determined by field and laboratory measurements. Dissolved oxygen sensors and pulse‐amplitude modulated (PAM) fluorometry were used for the measurements of photosynthetic efficiency. A Diving‐PAM revealed that underwater measurements of the effective quantum yield (Φ _PSII ) of both species declined with increasing incident PAR, with minimum Φ _PSII occurring during noon to early afternoon. Φ _PSII recovered in the evening, indicating photo‐adaptation to excessive PAR. In laboratory experiments, Φ _PSII also decreased under continuous exposure to 1000 μmol photons m^?2 s^?1; and full recovery occurred after 12 h of dark acclimatization. The net photosynthesis – PAR experiments of S. mcclurei and S. oligocystum conducted at 28°C revealed that the net photosynthetic rate quickly increased at PAR below the saturation irradiance of 361 and 301 μmol photons m^?2 s^?1 and nearly saturated to maximum net photosynthetic rates of 385 and 292 μg O₂ g_ww ^{? 1} min^?1 without photoinhibition, respectively. Gross photosynthesis and dark respiration experiments determined over a range of temperatures (12–40°C), revealed that the maximum gross photosynthetic rates of 201 and 147 μg O₂ g_ww ^{? 1} min^?1 occurred at 32.9 and 30.7°C for S. mcclurei and S. oligocystum, respectively. The dark respiration rates increased exponentially over the temperature ranges examined. The estimated maximum value of the maximum quantum yield occurred at 19.3 and 20.0°C and was 0.76 and 0.74, respectively. Similar to the natural habitat of the study site, these two species tolerated the relatively high temperatures and broad range of PAR. The ability of these species to recover from exposure to high PAR is one of the mechanisms that allow them to flourish in the shallow water environment.